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Title: Gene encoding plant asparagine synthetase

The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
Authors:
 [1] ;  [1]
  1. (New York, NY)
Publication Date:
OSTI Identifier:
868982
Report Number(s):
US 5256558
US patent application 07/514,816
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
encoding; plant; asparagine; synthetase; identification; cloning; plant; asparagine; synthetase; enzyme; involved; formation; asparagine; major; nitrogen; transport; compound; plants; described; expression; vectors; constructed; coding; sequence; utilized; produce; plant; engineer; herbicide; resistant; plants; salt; drought; tolerant; plants; pathogen; resistant; plants; dominant; selectable; marker; select; novel; herbicides; compounds; useful; agents; synchronize; plant; cells; culture; promoter; plant; directs; levels; expression; induced; organ; specific; manner; darkness; described; promoter; direct; expression; heterologous; coding; sequences; appropriate; hosts; compounds useful; coding sequences; plant cell; plant cells; resistant plants; resistant plants; coding sequence; coding sequence; expression vectors; expression vector; specific manner; plant asparagine; plant asparagine; asparagine synthetase; asparagine synthetase /435/999/