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Title: Integrated fluorescence analysis system

An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.
Authors:
 [1] ;  [1]
  1. (Los Alamos, NM)
Publication Date:
OSTI Identifier:
868318
Report Number(s):
US 5117466
Resource Type:
Patent
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Country of Publication:
United States
Language:
English
Subject:
integrated; fluorescence; analysis; integrated; fluorescence; analysis; enables; component; sample; virtually; sorted; sample; volume; spectrum; component; identified; fluorescence; spectrum; entire; sample; flow; cytometer; birefringent; optics; enables; entire; spectrum; resolved; set; representing; intensity; spectral; components; spectrum; spectral; components; selected; scanning; laser; microscope; preferably; confocal; microscope; whereby; spectrum; individual; pixels; voxels; sample; compared; individual; pixels; voxels; containing; selected; spectral; components; identified; image; formed; morphology; sample; respect; components; selected; spectral; components; physical; sorting; sample; components; obtain; morphological; information; selected spectral; selected spectral; confocal microscope; flow cytometer; spectral components; spectral components; spectral components; spectral components; scanning laser; sample volume; individual pixels; individual pixels; fluorescence spectrum; laser microscope; sample components; fluorescence analysis; fluorescence analysis; integrated fluorescence; integrated fluorescence; spectral component; spectral component; spectral component; spectral component /382/250/356/