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Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.
Authors:
 [1] ;  [2]
  1. (557 Escondido Cir., Livermore, CA 94550)
  2. (5178 Diane La., Livermore, CA 94550)
Publication Date:
OSTI Identifier:
863356
Report Number(s):
US 4155916
Resource Type:
Patent
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Country of Publication:
United States
Language:
English
Subject:
fluorescence; method; enzyme; analysis; couples; aromatic; amines; aromatic; aldehydes; analysis; proteinases; accomplished; conventional; amino; acid; containing; aromatic; amine; substrates; aromatic; amines; 4-methoxy-2-naphthylamine; 4m2na; 2-naphthylamine; aminoisophthalic; acid; dimethyl; ester; p-nitroaniline; 5-methoxy-1-aminofluorene; coumarin; derivatives; resulting; enzymatic; hydrolysis; substrate; couples; aromatic; aldehydes; 5-nitrosalicylaldehyde; 5-nsa; benzaldehyde; p-nitrobenzaldehyde; produce; schiff-base; complexes; water; insoluble; schiff-base; complexes; produce; shift; blue; orange-red; visible; fluorescence; complexes; useful; assay; enzymes; acid containing; aromatic amines; aromatic amines; amino acid; enzymatic hydrolysis; water insoluble; aromatic aldehydes; aromatic aldehydes; enzyme analysis; fluorescence method; coumarin derivatives; ethyl ester; methyl ester; aromatic amine; aromatic amine; aromatic amine; amine substrates; conventional amino; couples aromatic; containing aromatic /999/435/