NMR methods for in-situ biofilm metabolism studies: spatial and temporal resolved measurements
Journal Article
·
· Water Science and Technology
We are developing nuclear magnetic resonance (NMR) microscopy, spectroscopy and combined NMR/optical techniques to the study of biofilms. Objectives include: time and depth-resolved metabolite concentrations with isotropic spatial resolution on the order of 10 microns, metabolic pathways and flux rates, mass transport and ultimately their correlation with gene expression by optical microscopy in biofilms. These methods are being developed with Shewanella oneidensis MR-1 as a model system, but are equally applicable to other biofilm systems of interest. Thus, spatially resolved NMR of biofilms is expected to contribute significantly to the understanding of adherent cell metabolism.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 860407
- Report Number(s):
- PNNL-SA-43300; WSTED4; 10895; TRN: US200524%%176
- Journal Information:
- Water Science and Technology, Vol. 52, Issue 7; ISSN 0273-1223
- Country of Publication:
- United States
- Language:
- English
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