Systematic variation in gene expression patterns in human cancer cell lines
We used cDNA micro arrays to explore the variation in expression of approximately 8,000 unique genes among the 60 cell lines used in the National Cancer Institute s screen for anti-cancer drugs. Classification of the cell lines based solely on the observed patterns of gene expression revealed a correspondence to the ostensible origins of the tumors from which the cell lines were derived. The consistent relationship between the gene expression patterns and the tissue of origin allowed us to recognize outliers whose previous classification appeared incorrect. Specific features of the gene expression patterns appeared to be related to physiological properties of the cell lines, such as their doubling time in culture, drug metabolism or the interferon response. Comparison of gene expression patterns in the cell lines to those observed in normal breast tissue or in breast tumor specimens revealed features of the expression patterns in the tumors that had recognizable counterparts in specific cell lines, reflecting the tumor, stromal and inflammatory components of the tumor tissue. These results provided a novel molecular characterization of this important group of human cell lines and their relationships to tumors in vivo.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; National Cancer Institute Grant CA 077097; Howard Hughes Medical Institute, Walter and Idun Berry Fellow, Alfred P. Sloan Foundation Fellow, Life Science Research Foundation. SmithKline Beecham Pharmaceuticals Fellow
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 842486
- Report Number(s):
- LBNL-45751; R&D Project: GHEIS2; TRN: US200516%%55
- Journal Information:
- Nature Genetics, Vol. 24, Issue 3; Other Information: Journal Publication Date: March 2000
- Country of Publication:
- United States
- Language:
- English
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