A study of the first and second polar bodies in mouse oogenesis
- Institute of Molecular Biology and Genetics, Kiev (Ukraine)
The possibility of using a polar body for biopsy of human oocytes and early embryos has recently been shown. Genetic analysis of polar bodies can also provide additional information about the mechanisms underlying oocyte maturation. The first polar body is extremely unstable: in mouse oocytes, it disintegrates within several hours. Thus, the possibilities for its analysis are limited. We obtained karyoplasts of mouse eggs that contained the metaphase II spindle. By using them as a model for the first polar body, we studied the causes of its rapid disintegration. The rates of disintegration of the karyoplasts treated with various inhibitors of the cytoskeleton indicate that disintegration of the first polar body may be due to interaction between the actin cytoskeleton, chromatin, and the plasma membrane. The second polar body is found in mouse embryos until the blastocyst stage. Fusion of the second polar body with the enucleated zygote allowed analysis of its chromosomes. 22 refs., 5 figs., 1 tab.
- OSTI ID:
- 83444
- Journal Information:
- Russian Journal of Developmental Biology, Vol. 26, Issue 3; Other Information: PBD: May-Jun 1995; TN: Translated from Ontogenez; 26: No. 3, 196-200(1995)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Calcium and actin in the saga of awakening oocytes
MEIOSIS, MITOSIS, AND THE INDUCTION OF CHROMOSOMAL ABNORMALITIES IN HYMENOLEPIS DIMINUTA BY GAMMA RAYS