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Title: Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7

Abstract

The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5-flanking region at 1,780 1,287 bp, and at 611 to 208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226 percent by genotoxic 2-acetylaminofluorene and 347 percent by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells.

Authors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Director. Office of Science. Biological and Environmental Research; National Science Council of the Republic of China, Taiwan Grant NSC89-2320-B-006-008 (US)
OSTI Identifier:
824631
Report Number(s):
LBNL-52927
R&D Project: LGFGAA; TRN: US200419%%165
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Journal Of Biomedical Science
Additional Journal Information:
Journal Volume: 10; Other Information: Journal Publication Date: 2003; PBD: 15 Mar 2002
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; DOGS; EXONS; GENES; HISTONES; INTRONS; KIDNEYS; LUCIFERASE; MEMBRANES; NEOPLASMS; PROMOTERS; PROTEINS; SPLICING; MULTIDRUG RESISTANCE ABC TRANSPORTER MRP7 GENOMIC ORGANIZATION PROMOTER ANALYSIS

Citation Formats

Kao, Hsin-Hsin, Chang, Ming-Shi, Cheng, Jan-Fang, and Huang, Jin-Ding. Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7. United States: N. p., 2002. Web.
Kao, Hsin-Hsin, Chang, Ming-Shi, Cheng, Jan-Fang, & Huang, Jin-Ding. Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7. United States.
Kao, Hsin-Hsin, Chang, Ming-Shi, Cheng, Jan-Fang, and Huang, Jin-Ding. 2002. "Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7". United States.
@article{osti_824631,
title = {Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7},
author = {Kao, Hsin-Hsin and Chang, Ming-Shi and Cheng, Jan-Fang and Huang, Jin-Ding},
abstractNote = {The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5-flanking region at 1,780 1,287 bp, and at 611 to 208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226 percent by genotoxic 2-acetylaminofluorene and 347 percent by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells.},
doi = {},
url = {https://www.osti.gov/biblio/824631}, journal = {Journal Of Biomedical Science},
number = ,
volume = 10,
place = {United States},
year = {Fri Mar 15 00:00:00 EST 2002},
month = {Fri Mar 15 00:00:00 EST 2002}
}