Structural characterization of the reaction pathway in phosphoserine phosphatase: Crystallographic 'snapshots' of intermediate states.
Phosphoserine phosphatase (PSP) is a member of a large class of enzymes that catalyze phosphoester hydrolysis using a phosphoaspartate enzyme intermediate. PSP is a likely regulator of the steady-state-serine level in the brain, which is a critical co-agonist of the N-methyl--aspartate type of glutamate receptors. Here, we present high-resolution (1.5 1.9 Angstrom) structures of PSP from Methanococcus jannaschii, which define the open state prior to substrate binding, the complex with phosphoserine substrate bound (with a D to N mutation in the active site), and the complex with AlF3, a transition-state analog for the phospho-transfer steps in the reaction. These structures, together with those described for the BeF3- complex (mimicking the phospho-enzyme) and the enzyme with phosphate product in the active site, provide a detailed structural picture of the full reaction cycle. The structure of the apostate indicates partial unfolding of the enzyme to allow substrate binding, with refolding in the presence of substrate to provide specificity. Interdomain and active-site conformational changes are identified. The structure with the transition state analog bound indicates a ''tight'' intermediate. A striking structure homology, with significant sequence conservation, among PSP, P-type ATPases and response regulators suggests that the knowledge of the PSP reaction mechanism from the structures determined will provide insights into the reaction mechanisms of the other enzymes in this family.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- National Institutes of Health (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 823452
- Report Number(s):
- LBNL-50155; JMOBAK; R&D Project: 864A11; TRN: US200415%%498
- Journal Information:
- Journal of Molecular Biology, Vol. 319, Issue 2; Other Information: Journal Publication Date: 2002; PBD: 12 Apr 2004; ISSN 0022-2836
- Country of Publication:
- United States
- Language:
- English
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Crystal structure of phosphoserine phosphatase from Methanococcusjannaschii, a hyperthermophile, at 1.8 Angstrom resolution
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Related Subjects
BRAIN
CONFORMATIONAL CHANGES
ENZYMES
HYDROLYSIS
MUTATIONS
PHOSPHATASES
PHOSPHATES
REACTION KINETICS
SPECIFICITY
SUBSTRATES
PHOSPHOTRANSFERASES
PHOSPHOTRANSFER PHOSPHO-MONOESTER HYDROLYSIS PHOSPHO-ASPARTYL ENZYME INTERMEDIATE PHOSPHOSERINE PHOSPHATASE ASSOCIATIVE MECHANISM DISSOCIATIVE MECHANISM