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Title: Yakima/Klickitat Fisheries Project Genetic Studies; Yakima/Klickitat Fisheries Project Monitoring and Evaluation, 2000-2001 Annual Report.

Genetic work for 2001 consisted of two major phases, both reported on here. The first is a DNA microsatellite analysis of several hundred juveniles from the experimental spawning channel at the Cle Elum Supplementation Research Facility, using the genetic markers to assign the juveniles to parents, and thus judge reproductive success of individual fish. The second is a reevaluation and revision of plans for studying domestication in the spring chinook supplementation effort. The pedigree analysis was significant in three respects. First, it showed that this approach can be successfully applied to the spawning channel research. Secondly it showed that this approach does indeed yield very useful information about the relative reproductive success of fish in the channel. Finally, it showed that this information can yield additional information about the experimental design. Of the 961 juveniles on which analysis was attempted, 774 yielded enough genetic information to be used in the pedigree analysis. Of these, 754 were assigned to males and females known to have been placed into the channel. Of the other 20, all were assignable to females, but sires were unknown. The genotypes of 17 of these were consistent with a single theoretical male genotype, suggesting a single precocialmore » male sired them. The inferred parentage of the fish demonstrated that there had been substantial leakage of juveniles from one section of the channel into another. Reproductive success of females was fairly even, but success of males varied considerably. In a group of seven males (including the hypothetical one), one contributed 79% of the progeny analyzed, and three contributed none. The domestication experimental design evaluation was prompted by a critical review of the project by the Independent Scientific Review Panel (ISRP). The ISRP review set into motion a design revision process which extended beyond the contract period; the report presented here is intended to be an account of our work through the end of the contract period, so does not include developments beyond that point. As such, combined with the upcoming 2002 report, it will provide a complete record of our process through the experimental design revision process. The current report contains the following: (1) An explanation of the general concept of domestication, and why domestication is a concern in the YKFP spring chinook program; (2) A discussion of the basics of experimental design for domestication; (3) A history of domestication experimental design for domestication in the YKFP; (4) A review of potential designs that would answer the ISRP's criticisms; (5) A revised design containing the following elements--A control line under continuous hatchery culture (i.e.; no spawning in the wild); use of the Naches population, where appropriate, as a wild control line; (6) Cryopreservation of sperm for later evaluation of long-term genetic trend; and (7) Continuous monitoring of phenotypic trend in the supplemented line.« less
Authors:
; ;  [1]
  1. (Washington Department of Fish and Wildlife, Olympia, WA)
Publication Date:
OSTI Identifier:
819777
Report Number(s):
DOE/BP-00004666-2
R&D Project: 199506325 ; 199506424; TRN: US200324%%21
DOE Contract Number:
00004666 ; 00013756
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Nov 2002
Research Org:
Bonneville Power Administration, Portland, OR (US)
Sponsoring Org:
US Department of Energy (US)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; DESIGN; DNA; EVALUATION; FEMALES; FISHERIES; GENETICS; GENOTYPE; JUVENILES; MALES; MONITORING; PROGENY; SPERMATOZOA SALMON - GENETICS; FISHERY RESOURCES - HATCHERY VS. WILD STOCKS - WASHINGTON (STATE) - YAKIMA RIVER WATERSHED