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Title: High resolution mapping of genetic factors affecting abdominal bristle number in Drosophila melanogaster

Abstract

Factors responsible for selection response for abdominal bristle number and correlated responses in sternopleural bristle number were mapped to the X and third chromosome of Drosophila melanogaster. Lines divergent for high and low abdominal bristle number were created by 25 generations of artificial selection from a large base population, with an intensity of 25 individuals of each sex selected from 100 individuals of each sex scored per generation. Isogenic chromosome substitution lines in which the high (H) X or third chromosome were placed in an isogenic low (L) background were derived from the selection lines and from the 93 recombinant isogenic (RI) HL X and 67 RI chromosome 3 lines constructed from them. Highly polymorphic neutral roo transposable elements were hybridized in situ to the polytene chromosomes of the RI lines to create a set of cytogenetic markers. These techniques yielded a dense map with an average spacing of 4 cM between informative markers. Two factors with large effects on abdominal bristle number were mapped on the X chromosome and five factors on the third chromosome. One factor with a large effect on sternopleural bristle number was mapped to the X and two were mapped to the third chromosome; allmore » factors with sternopleural effects corresponded to those with effects on abdominal bristle number. Two of the chromosome 3 factors with large effects on abdominal bristle number were also associated with reduced viability. Significant sex-specific effects and epistatic interactions between mapped factors of the same order of magnitude as the additive effects were observed. All factors mapped to the approximate positions of likely candidate loci previously characterized by mutations with large effects on bristle number. 55 refs., 4 figs., 7 tabs.« less

Authors:
 [1]; ;  [1]; ; ;  [2]
  1. Univ. of California, Davis, CA (United States)
  2. North Carolina State Univ., Raleigh, NC (United States)
Publication Date:
OSTI Identifier:
75593
Resource Type:
Journal Article
Journal Name:
Genetics
Additional Journal Information:
Journal Volume: 139; Journal Issue: 3; Other Information: PBD: Mar 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENETIC MAPPING; ALGORITHMS; RESOLUTION; CHROMOSOMES; DNA HYBRIDIZATION; X CHROMOSOME; DROSOPHILA; BIOLOGICAL MARKERS; GENES; GENE MUTATIONS; GENETIC EFFECTS; SEX DEPENDENCE

Citation Formats

Long, A D, McMaster Univ., Ontario, Mullaney, S L, Langley, C H, Reid, L A, Fry, J D, and Mackay, T F.C. High resolution mapping of genetic factors affecting abdominal bristle number in Drosophila melanogaster. United States: N. p., 1995. Web.
Long, A D, McMaster Univ., Ontario, Mullaney, S L, Langley, C H, Reid, L A, Fry, J D, & Mackay, T F.C. High resolution mapping of genetic factors affecting abdominal bristle number in Drosophila melanogaster. United States.
Long, A D, McMaster Univ., Ontario, Mullaney, S L, Langley, C H, Reid, L A, Fry, J D, and Mackay, T F.C. 1995. "High resolution mapping of genetic factors affecting abdominal bristle number in Drosophila melanogaster". United States.
@article{osti_75593,
title = {High resolution mapping of genetic factors affecting abdominal bristle number in Drosophila melanogaster},
author = {Long, A D and McMaster Univ., Ontario and Mullaney, S L and Langley, C H and Reid, L A and Fry, J D and Mackay, T F.C.},
abstractNote = {Factors responsible for selection response for abdominal bristle number and correlated responses in sternopleural bristle number were mapped to the X and third chromosome of Drosophila melanogaster. Lines divergent for high and low abdominal bristle number were created by 25 generations of artificial selection from a large base population, with an intensity of 25 individuals of each sex selected from 100 individuals of each sex scored per generation. Isogenic chromosome substitution lines in which the high (H) X or third chromosome were placed in an isogenic low (L) background were derived from the selection lines and from the 93 recombinant isogenic (RI) HL X and 67 RI chromosome 3 lines constructed from them. Highly polymorphic neutral roo transposable elements were hybridized in situ to the polytene chromosomes of the RI lines to create a set of cytogenetic markers. These techniques yielded a dense map with an average spacing of 4 cM between informative markers. Two factors with large effects on abdominal bristle number were mapped on the X chromosome and five factors on the third chromosome. One factor with a large effect on sternopleural bristle number was mapped to the X and two were mapped to the third chromosome; all factors with sternopleural effects corresponded to those with effects on abdominal bristle number. Two of the chromosome 3 factors with large effects on abdominal bristle number were also associated with reduced viability. Significant sex-specific effects and epistatic interactions between mapped factors of the same order of magnitude as the additive effects were observed. All factors mapped to the approximate positions of likely candidate loci previously characterized by mutations with large effects on bristle number. 55 refs., 4 figs., 7 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/75593}, journal = {Genetics},
number = 3,
volume = 139,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 1995},
month = {Wed Mar 01 00:00:00 EST 1995}
}