The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations
- Semenov Institute of Chemical Physics, Moscow (Russian Federation)
- Russian Medical State Univ., Moscow (Russian Federation)
Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G{sub 1} phase (G{sub 1} process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G{sub 1} process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G{sub 1} process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G{sub 1} process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed. 18 refs., 3 figs., 2 tabs.
- OSTI ID:
- 75554
- Journal Information:
- Russian Journal of Genetics, Vol. 31, Issue 4; Other Information: PBD: Apr 1995; TN: Translated from Genetika; 31: No. 4, 485-491(1995)
- Country of Publication:
- United States
- Language:
- English
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