Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro

Mukherjee, D; Guha, D; Kumar, V

Title: Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro

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Abstract

Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.

Authors:

Mukherjee, D; Guha, D; Kumar, V ^[1]

Department of Zoology, University of Kalyani (India)

Publication Date:: Mon Jun 01 00:00:00 EDT 1992

OSTI Identifier:: 7262686

Resource Type:: Journal Article

Journal Name:: Biomedical and Environmental Sciences; (United States)

Additional Journal Information:: Journal Volume: 5:2; Journal ID: ISSN 0895-3988

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CADMIUM; TOXICITY; CHOLESTEROL; BIOSYNTHESIS; FISHES; SENSITIVITY; MERCURY CHLORIDES; OXIDOREDUCTASES; ENZYME ACTIVITY; PHENOL; SULFIDES; GONADOTROPINS; INHIBITION; OOCYTES; OVARIES; WATER POLLUTION; ANIMALS; AQUATIC ORGANISMS; AROMATICS; BODY; CHALCOGENIDES; CHLORIDES; CHLORINE COMPOUNDS; ELEMENTS; ENZYMES; FEMALE GENITALS; GERM CELLS; GONADS; HALIDES; HALOGEN COMPOUNDS; HORMONES; HYDROXY COMPOUNDS; MERCURY COMPOUNDS; MERCURY HALIDES; METALS; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHENOLS; PITUITARY HORMONES; POLLUTION; PROTEINS; STEROIDS; STEROLS; SULFUR COMPOUNDS; SYNTHESIS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

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                    Mukherjee, D, Guha, D, and Kumar, V. Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro.  United States: N. p., 1992. 
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                    Mukherjee, D, Guha, D, & Kumar, V. Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro.  United States.

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                    Mukherjee, D, Guha, D, and Kumar, V. 1992.  
        "Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro".  United States.

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@article{osti_7262686,

  title        = {Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro},

  author       = {Mukherjee, D and Guha, D and Kumar, V},

  abstractNote = {Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/7262686},
  journal      = {Biomedical and Environmental Sciences; (United States)},

  issn         = {0895-3988},

  number       = ,

  volume       = 5:2,

  place        = {United States},

  year         = {Mon Jun 01 00:00:00 EDT 1992},

  month        = {Mon Jun 01 00:00:00 EDT 1992}

}

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