Regulation of mitogen-stimulated human T-cell proliferation, interleukin-2 production, and interleukin-2 receptor expression by protein kinase C inhibitor, H-7
- Kansas State Univ., Manhattan (United States)
- Argonne National Lab., IL (United States)
Recently published reports suggest that the activation of protein kinase C (PKC) plays an important role in the activation pathway of many cell types. In this study, the authors examined the role of PKC in human T-cell proliferation, IL-2 production, and IL-2R expression, when cultured with the mitogen PHA, the PKC inhibitor H-7, and H-7 control HA1004. H-7 inhibited the PHA-simulated ({sup 3}H)thymidine uptake, IL-2production, and IL-2R expression in a dose-related manner. Further, they found H-7 inhibited T-cell proliferation, IL-2 production, and IL-2mRNA from PHA plus PMA-stimulated cultures. They also found that H-7 inhibited the early-stage activation of PHA-stimulated cells. The presence of exogenous purified human IL-2 or rIL-4 partly reversed the immunosuppression caused by H-7. In contrast, HA1004 had no effect on cell proliferation, IL-2 production, or IL-2R expression. The results demonstrate that PKC activation is one major pathway through which T-cells become activated.
- OSTI ID:
- 7261120
- Journal Information:
- Cellular Immunology; (United States), Vol. 129; ISSN 0008-8749
- Country of Publication:
- United States
- Language:
- English
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ENZYME INHIBITORS
BIOCHEMICAL REACTION KINETICS
LYMPHOCYTES
CELL PROLIFERATION
LYMPHOKINES
BIOSYNTHESIS
PHOSPHOTRANSFERASES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
DOSE-RESPONSE RELATIONSHIPS
MITOGENS
RECEPTORS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
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BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ENZYMES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SOMATIC CELLS
SYNTHESIS
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550200* - Biochemistry