Expanding the genetic variability of flatpea using tissues culture, mutagenesis, and intercrossing techniques
Flatpea (Lathyrus sylvestris L.) is a potentially valuable forage legume but contains high levels of 2,4-diaminobutyric acid (DABA), a compound that can have adverse effects on some animals, including rats and poultry. To increase genetic variability in foliar DABA content and other traits of interest, three approaches were utilized: (1) regeneration of flatpea plants from tissue culture to produce potential somaclonal variants, (2) seed irradiation and screening of potentially mutated progeny, and (3) intercrossing among flatpea accessions. Low-frequency whole plant regeneration of flatpea was obtained from hypocotyl-derived callus cultures. Initial tests established that the effective range of gamma-irradiation for seed treatment was between 10.0 and 17.5 kR. Within this range, reduction in percentage of both seedling height and plant survival was a linear function of dose. Individual M{sub 2} plants that contained reduced levels of DABA were identified. No significant trend in DABA concentration with increasing gamma irradiation was apparent. Flatpea pollination methods were evaluated prior to utilization of intercrossing for inducing genetic variability. Appropriate flower stages for emasculation were determined by in vitro germination of pollen. Lines that produced high numbers of seeds per pollination were identified by crossing in all possible combinations among seven flatpea accessions.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA)
- OSTI ID:
- 7254534
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINOBUTYRIC ACID
BIOSYNTHESIS
LEGUMINOSAE
GENETIC RADIATION EFFECTS
MUTATIONS
RADIOINDUCTION
BIOLOGICAL REGENERATION
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
GENETIC VARIABILITY
MUTAGENESIS
SEEDS
SURVIVAL TIME
TISSUE CULTURES
AMINO ACIDS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
DRUGS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
SYNTHESIS
560140* - Radiation Effects on Plants