17 beta-hydroxysteroid dehydrogenase activity in canine pancreas

Journal Article · Fri Apr 15 00:00:00 EDT 1988 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(88)80724-1· OSTI ID:7253557

Mendoza-Hernandez, G; Lopez-Solache, I; Rendon, J L; Diaz-Sanchez, V; Diaz-Zagoya, J C

The mitochondrial fraction of the dog pancreas showed NAD(H)-dependent enzyme activity of 17 beta-hydroxysteroid dehydrogenase. The enzyme catalyzes oxidoreduction between androstenedione and testosterone. The apparent Km value of the enzyme for androstenedione was 9.5 +/- 0.9 microM, the apparent Vmax was determined as 0.4 nmol mg-1 min-1, and the optimal pH was 6.5. In phosphate buffer, pH 7.0, maximal rate of androstenedione reduction was observed at 37 degrees C. The oxidation of testosterone by the enzyme proceeded at the same rate as the reduction of the androstenedione at a pH of 6.8-7.0. The apparent Km value and the optimal pH of the enzyme for testosterone were 3.5 +/- 0.5 microM and 7.5, respectively.

• Research Organization: Universidad Nacional Autonoma de Mexico, D. F. (Mexico)
• OSTI ID: 7253557
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 152:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
ENZYME ACTIVITY
ANDROSTENEDIONE
BIOCHEMICAL REACTION KINETICS
DOGS
MITOCHONDRIA
PANCREAS
REDOX REACTIONS
TESTOSTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ENZYMES
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
REACTION KINETICS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques