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Title: Hematopoietic microenvironment. Origin, lineage, and transplantability of the stromal cells in long-term bone marrow cultures from chimeric mice

Abstract

Studies of bone marrow transplant patients have suggested that the stromal cells of the in vitro hematopoietic microenvironment are transplantable into conditioned recipients. Moreover, in patients with myeloproliferative disorders, all of the stromal cells, which include presumptive endothelial cells, appear to be derived from hematopoietic precursors. To confirm these findings, we have constructed two chimeric mouse models: (a) traditional radiation chimeras, and (b) fetal chimeras, produced by placental injection of bone marrow into genetically anemic Wx/Wv fetuses, a technique that essentially precludes engraftment of nonhematopoietic cells. Using two-color indirect immunofluorescence, the stromal cells in long-term bone marrow culture derived from these chimeras were analyzed for donor or host origin by strain-specific H-2 antigens, and for cell lineage by a variety of other specific markers. 75-95% of the stromal cells were shown to be hematopoietic cells of the monocyte-macrophage lineage, based upon donor origin, phagocytosis, and expression of specific hematopoietic surface antigens. The remaining 5-25% of the stromal cells were exclusively host in origin. Apart from occasional fat cells, these cells uniformly expressed collagen type IV, laminin, and a surface antigen associated with endothelial cells. Since these endothelial-like cells are not transplantable into radiation or fetal chimeras, they are not derivedmore » from hematopoietic stem cells. The contrast between our findings and human studies suggests either unexpected species differences in the origin of stromal lineages or limitations in the previous methodology used to detect nonhematopoietic stromal cells.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of Texas Health Science Center, Dallas (USA)
OSTI Identifier:
7252588
Resource Type:
Journal Article
Journal Name:
J. Clin. Invest.; (United States)
Additional Journal Information:
Journal Volume: 81:4
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BONE MARROW CELLS; TRANSPLANTS; HEMATOPOIETIC SYSTEM; ADIPOSE TISSUE; ANTIGENS; BIOLOGICAL MODELS; CELL CULTURES; ENDOTHELIUM; FETUSES; MACROPHAGES; MICE; MONOCYTES; PHAGOCYTOSIS; RADIATION CHIMERAS; STEM CELLS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHIMERAS; CONNECTIVE TISSUE; CONNECTIVE TISSUE CELLS; LEUKOCYTES; MAMMALS; MATERIALS; MOSAICISM; PHAGOCYTES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Perkins, S, and Fleischman, R A. Hematopoietic microenvironment. Origin, lineage, and transplantability of the stromal cells in long-term bone marrow cultures from chimeric mice. United States: N. p., 1988. Web. doi:10.1172/JCI113419.
Perkins, S, & Fleischman, R A. Hematopoietic microenvironment. Origin, lineage, and transplantability of the stromal cells in long-term bone marrow cultures from chimeric mice. United States. https://doi.org/10.1172/JCI113419
Perkins, S, and Fleischman, R A. 1988. "Hematopoietic microenvironment. Origin, lineage, and transplantability of the stromal cells in long-term bone marrow cultures from chimeric mice". United States. https://doi.org/10.1172/JCI113419.
@article{osti_7252588,
title = {Hematopoietic microenvironment. Origin, lineage, and transplantability of the stromal cells in long-term bone marrow cultures from chimeric mice},
author = {Perkins, S and Fleischman, R A},
abstractNote = {Studies of bone marrow transplant patients have suggested that the stromal cells of the in vitro hematopoietic microenvironment are transplantable into conditioned recipients. Moreover, in patients with myeloproliferative disorders, all of the stromal cells, which include presumptive endothelial cells, appear to be derived from hematopoietic precursors. To confirm these findings, we have constructed two chimeric mouse models: (a) traditional radiation chimeras, and (b) fetal chimeras, produced by placental injection of bone marrow into genetically anemic Wx/Wv fetuses, a technique that essentially precludes engraftment of nonhematopoietic cells. Using two-color indirect immunofluorescence, the stromal cells in long-term bone marrow culture derived from these chimeras were analyzed for donor or host origin by strain-specific H-2 antigens, and for cell lineage by a variety of other specific markers. 75-95% of the stromal cells were shown to be hematopoietic cells of the monocyte-macrophage lineage, based upon donor origin, phagocytosis, and expression of specific hematopoietic surface antigens. The remaining 5-25% of the stromal cells were exclusively host in origin. Apart from occasional fat cells, these cells uniformly expressed collagen type IV, laminin, and a surface antigen associated with endothelial cells. Since these endothelial-like cells are not transplantable into radiation or fetal chimeras, they are not derived from hematopoietic stem cells. The contrast between our findings and human studies suggests either unexpected species differences in the origin of stromal lineages or limitations in the previous methodology used to detect nonhematopoietic stromal cells.},
doi = {10.1172/JCI113419},
url = {https://www.osti.gov/biblio/7252588}, journal = {J. Clin. Invest.; (United States)},
number = ,
volume = 81:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1988},
month = {Fri Apr 01 00:00:00 EST 1988}
}