Phorbol esters modulate cyclic AMP accumulation in porcine thyroid cells

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90377-3· OSTI ID:7252535

Emoto, T; Kasai, K; Hiraiwa, M; Shimoda, S

In cultured porcine thyroid cells, during 60 min incubation phorbol 12-myristate 13-acetate (PMA) had no effect on basal cyclic AMP accumulation and slightly stimulated cyclic AMP accumulation evoked by thyroid stimulating hormone (TSH) or forskolin. Cholera toxin-induced cyclic AMP accumulation was significantly stimulated by PMA. On the other hand, cyclic AMP accumulation evoked by prostaglandin E/sub 1/ or E/sub 2/ (PGE/sub 1/ and PGE/sub 2/) was markedly depressed by simultaneous addition of PMA. These opposing effects of PMA on cyclic AMP accumulation evoked by PGE and cholera toxin were observed in a dose-related fashion, with half-maximal effect of around 10/sup -9/ M in either case. The almost same effects of PMA on cyclic AMP accumulation in basal and stimulated conditions were also observed in freshly prepared thyroid cells. The present study was performed in the presence of phosphodiesterase inhibitor, 3-iso-butyl-1-methylxanthine (IBMX), indicating that PMA affected adenylate cyclase activity. Therefore, it is suggested that PMA may modulate the production of cyclic AMP in response to different stimuli, possibly by affecting several sites in the adenylate cyclase complex in thyroid cells.

• Research Organization: Dokkyo Univ. School of Medicine, Tochigi (Japan)
• OSTI ID: 7252535
• Journal Information: Life Sci.; (United States), Vol. 42:22
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
BIOLOGICAL ACCUMULATION
CYCLASES
ENZYME ACTIVITY
PHORBOL ESTERS
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
PROSTAGLANDINS
SWINE
THYROID CELLS
TOXINS
TSH
ANIMAL CELLS
ANIMALS
ANTIGENS
CARCINOGENS
DOMESTIC ANIMALS
ENZYMES
ESTERS
HORMONES
LYASES
MAMMALS
MATERIALS
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
TOXIC MATERIALS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology