The pharmacological characterization of the bicarbonate, proton, and mucus transport systems of the rodent gastric mucosa and their significance in cytoprotection

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7246103

Casciano, C N

The ability of 2-methyl-8-(phenyl-methoxy) imidazo (1,2{alpha}) pyridine-3-acetonitrile (MPIPA) to inhibit acid secretion and to promote a HCO{sub 3}{sup {minus}} secretion response was examined. MPIPA at 5 {times} 10{sup {minus}6} M inhibited histamine, methacholine, and db{prime} cAMP stimulated acid secretion. MPIPA induced a dose-dependent increase in HCO{sub 3}{sup {minus}} secretion at doses from 100{sup {minus}6} to 10{sup {minus}3} M in both isolated gastric mucosa and fundic slice preparations. An evaluation of ion flux responses associated with the secretion of HCO{sub 3}{sup {minus}} induced by MPIPA revealed a high positive correlation between assayed HCO{sub 3}{sup {minus}} and K{sup +} in the luminal medium of isolated gastric mucosa. Using autoradiography {sup 14}C-MPIPA was localized in both surface epithelia cells and parietal cells within the gastric mucosa, which is consistent with the observed dual action of the compound.

• Research Organization: Rutgers-the State Univ., Newark, NJ (USA). Dept. of Chemistry
• OSTI ID: 7246103
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACETONITRILE
BIOCHEMICAL REACTION KINETICS
ACID CARBONATES
SECRETION
AUTORADIOGRAPHY
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CHOLINE
DOSE-RESPONSE RELATIONSHIPS
GASTROINTESTINAL TRACT
HISTAMINE
INHIBITION
MUCOUS MEMBRANES
PROTONS
RODENTS
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AZOLES
BARYONS
CHEMISTRY
DIGESTIVE SYSTEM
DRUGS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMIDAZOLES
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANES
NITRILES
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUATERNARY COMPOUNDS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques