Vanadate stimulation of IGF binding to rat adipocytes

Journal Article · Wed Jul 16 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)90262-7· OSTI ID:7245147

Kadota, S; Fantus, I G; Hersh, B; Posner, B I

Preincubation of adipocytes with insulin (10 ng/ml) stimulated binding of IGF-II to maximal levels of 160% above controls. Vanadate also augmented IGF-II binding with an increase of 126% above controls at a concentration of 1 mM. Coincubation of vanadate (1 mM) with a maximal stimulatory dose of insulin (10 ng/ml) produced no additive effect. However, at submaximal doses of insulin (0.1 ng/ml) the effect of vanadate was additive. Amiloride, a potent inhibitor of the insulin receptor kinase, inhibited the effects of both vanadate and insulin. The data are consistent with an effect of vanadate via a similar sequence of steps to that of insulin; perhaps involving activation of the insulin receptor kinase.

• Research Organization: McGill Univ., Montreal, Quebec
• OSTI ID: 7245147
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 138:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
FAT CELLS
RECEPTORS
VANADATES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
EXPERIMENTAL DATA
INCUBATION
INSULIN
PHOSPHOTRANSFERASES
RATS
RESPONSE MODIFYING FACTORS
STIMULATION
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
DATA
ENZYMES
HORMONES
INFORMATION
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry