Brain cholinergic involvement in the diurnal variations of the rapid development of tolerance to the hypothermic effect of ethanol

Conference · Wed Feb 26 00:00:00 EST 1992 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:7235737

Wang, Q H; Soliman, K F.A. ^[1]

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1. Florida A and M Univ., Tallahassee (United States)

Male Sprague-Dawley rats maintained under controlled environmental conditions were used. Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities were determined in the hypothalamus, pons, medulla oblongata, thalamus, midbrain, cerebral cortex, cerebellum and hippocampus of control rats and rats treated with ethanol either after a single dose at 10:00 and 22:00, or after a second dose administered 24hrs later at the same time schedules. Results of this experiment indicate that repeated administration with ethanol was associated with rapid development of tolerance to the hypothermic action of ethanol. A single injection of ethanol at 10:00 resulted in significant increase in ChAT activity of pons and cerebellum and decline of ChAT activity of midbrain. There were no significant changes in AChE activity at all of these different brain regions. A single injection of ethanol at 22:00 resulted in significant decrease in ChAT activity of the hypothalamus, pons, midbrain, hippocampus. At the same time, there was a significant decline of AChE activity of the pons, medulla and midbrain. These findings indicate that changes in the responsiveness of the brain cholinergic enzymes may explain the increase in hypothermia and the rapid development of tolerance.

• OSTI ID: 7235737
• Report Number(s): CONF-9204141-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 6:4; Conference: Federation of American Societies for Experimental Biology (FASEB) meeting, Anaheim, CA (United States), 5-9 Apr 1992; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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