Charge-state shifting of individual multiply-charged ions of bovine albumin dimer and molecular weight determination using an individual-ion approach

Journal Article · Fri Jul 01 00:00:00 EDT 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00085a025· OSTI ID:7201230

Cheng, X; Bakhtiar, R; Van Orden, S; Smith, R D ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States)

Ion-molecule reactions of individual multiply-protonated ions of bovine albumin dimer, formed from electrospray ionization, have been studied using a Fourier transform ion cyclotron resonance mass spectrometer. Upon reaction of ammonia with a group of individual ions, charge-state shifting was observed due to proton transfer. Repeated additions of ammonia during remeasurements of the same ion population were observed to induce multiple-step charge-state shifts. Charge-state-dependent reactivity, as well as nonstatistical behavior in reactivity, was observed due to the small ion population. The molecular weights of individual ions whose charge state shifted during reaction were determined with an accuracy of 67 ppm, the first example of using an individual-ion approach to the determination of molecular weight for a large biopolymer. The molecular weight distribution of a group of ions can be determined with a precision related to the number of ions examined and the weight heterogeneity of the sample. We obtained the molecular weight for eight individual ions from which a molecular weight of 133 320 [+-] 210 Da was calculated for bovine albumin dimer. 17 refs., 3 figs., 1 tab.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7201230
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 66:13; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ALBUMINS
MOLECULAR WEIGHT
IONS
TRAPPING
AMMONIA
DIMERS
IONIZATION
MASS SPECTROMETERS
CHARGED PARTICLES
HYDRIDES
HYDROGEN COMPOUNDS
MEASURING INSTRUMENTS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
ORGANIC COMPOUNDS
PROTEINS
SPECTROMETERS
550200* - Biochemistry
400102 - Chemical & Spectral Procedures