Batchwise synthesis of dihydroneopterin triphosphate with recycling of guanosine triphosphate cyclohydrolase I

Conference · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7196521

Ferre, J; Naylor, E W; Jacobson, K B

Synthesis of NH/sub 2/P/sub 3/ in the ultrafiltration cell allows continuous utilization of GCH I, thus yielding a much higher production of NH/sub 2/P/sub 3/ than with other traditional methods. The procedure takes advantage of the high stability of the E. coli enzyme, since typically the same enzyme batch is used for two or more weeks at 42/sup 0/C. The purity of the so synthesized NH/sub 2/P/sub 3/ is satisfactory for utilization with enzymes of the pteridine pathway. However, if separation of NH/sub 2/P/sub 3/ from buffer components is desired, it can be accomplished using silica-bonded cartridges with a separation protocol that takes advantage of the highly negative charge density of the NH/sub 2/P/sub 3/ molecule.

• Research Organization: Martin Marietta Energy Systems, Inc., Oak Ridge, TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7196521
• Report Number(s): CONF-880361-2; ON: DE88012202
• Resource Relation: Conference: Annual international pteridine workshop, Snowbird, UT, USA, 13 Mar 1988; Other Information: Paper copy only, copy does not permit microfiche production
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHATASES
SYNTHESIS
PTERIDINES
BIOSYNTHESIS
ENZYMES
ESCHERICHIA COLI
GUANOSINE
HYDROLASES
ULTRAFILTRATION
AROMATICS
AZAARENES
BACTERIA
ESTERASES
FILTRATION
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RIBOSIDES
SEPARATION PROCESSES
550200* - Biochemistry