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Title: Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: Inactivation of specific transcription factors

The inhibition of transcription by RNA polymerase III in poliovirus-infected cells was studied. Experiments utilizing two different cell lines showed that the initiation step of transcription by RNA polymerase III was impaired by infection of these cells with the virus. The observed inhibition of transcription was not due to shut-off of host cell protein synthesis by poliovirus. Among four distinct components required for accurate transcription in vitro from cloned DNA templates, activities of RNA polymerase III and transcription factor TFIIIA were not significantly affected by virus infection. The activity of transcription factor TFIIIC, the limiting component required for transcription of RNA polymerase III genes, was severely inhibited in infected cells, whereas that of transcription factor TFIIIB was inhibited to a lesser extent. The sequence-specific DNA-binding of TFIIIC to the adenovirus VA1 gene internal promoted, however, was not altered by infection of cells with the virus. The authors conclude that (i) at least two transcription factors, TFIIIB and TFIIIC, are inhibited by infection of cells with poliovirtus, (ii) inactivation of TFIIIC does not involve destruction of its DNA-binding domain, and (iii) sequence-specific DNA binding by TFIIIC may be necessary but is not sufficient for the formation of productive transcription complexes.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
7196494
Resource Type:
Journal Article
Resource Relation:
Journal Name: Mol. Cell. Biol.; (United States); Journal Volume: 7:11
Research Org:
Dept. of Microbiology and Immunology and Jonsson Comprehensive Cancer Center, Univ. of California, Los Angeles, School of Medicine, and the Dept. of Microbiology and Molecular Biology Institute, Univ. of California, Los Angeles, CA 90024 (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL TRANSFORMATIONS; MOLECULAR BIOLOGY; NUCLEOPROTEINS; POLIO VIRUS; RNA POLYMERASES; ENZYME INHIBITORS; TRANSCRIPTION; BIOLOGICAL PATHWAYS; ADENOVIRUS; CELL CULTURES; DNA SEQUENCING; EXPERIMENTAL DATA; IN VITRO; SPECIFICITY; DATA; ENZYMES; INFORMATION; MICROORGANISMS; NUCLEOTIDYLTRANSFERASES; NUMERICAL DATA; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; VIRUSES 550200* -- Biochemistry; 550700 -- Microbiology; 550900 -- Pathology