gamma. sub 2 -MSH immunoreactivity in the human heart
Abstract
In patients undergoing aorto-coronary by-pass surgery, we found a 26% arterial-venous difference of immunoreactive {gamma}{sub 2}-melanocytostimulating hormone (MSH), a proopiomelanocortin (POMC) derived peptide known to possess profound hemodynamic effects. These results prompted an investigation of the presence of {gamma}{sub 2}-MSH in the human heart. Using a two-step extraction procedure, regions of human hearts were examined by sensitive and specific radioimmunoassays to determine their {gamma}{sub 2}-MSH content. Mean ({plus minus} SEM) concentrations of 0.14 {plus minus} 0.023 pmol/g and 0.12 {plus minus} 0.017 were found in right atrium and right ventricle, respectively. High performance liquid chromatography indicated that 80-90 % of the total immunoreactivity eluted in a single sharp peak in a position identical to that of synthetic {gamma}{sub 2}-MSH.
- Authors:
-
- Univ. of Lund (Sweden)
- Publication Date:
- OSTI Identifier:
- 7195035
- Resource Type:
- Journal Article
- Journal Name:
- Life Sciences; (USA)
- Additional Journal Information:
- Journal Volume: 45:9; Journal ID: ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CARDIOVASCULAR DISEASES; BIOLOGICAL MARKERS; HEART; IMMUNE REACTIONS; PEPTIDES; BIOSYNTHESIS; LIQUID COLUMN CHROMATOGRAPHY; MAN; PATIENTS; RADIOIMMUNOASSAY; SURGERY; ANIMALS; BIOASSAY; BODY; CARDIOVASCULAR SYSTEM; CHROMATOGRAPHY; DIAGNOSTIC TECHNIQUES; DISEASES; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; MEDICINE; ORGANIC COMPOUNDS; ORGANS; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; SEPARATION PROCESSES; SYNTHESIS; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 550901 - Pathology- Tracer Techniques
Citation Formats
Ekman, R, Bjartell, A, Lisander, J, and Edvinsson, L. gamma. sub 2 -MSH immunoreactivity in the human heart. United States: N. p., 1989.
Web. doi:10.1016/0024-3205(89)90171-9.
Ekman, R, Bjartell, A, Lisander, J, & Edvinsson, L. gamma. sub 2 -MSH immunoreactivity in the human heart. United States. https://doi.org/10.1016/0024-3205(89)90171-9
Ekman, R, Bjartell, A, Lisander, J, and Edvinsson, L. 1989.
"gamma. sub 2 -MSH immunoreactivity in the human heart". United States. https://doi.org/10.1016/0024-3205(89)90171-9.
@article{osti_7195035,
title = {gamma. sub 2 -MSH immunoreactivity in the human heart},
author = {Ekman, R and Bjartell, A and Lisander, J and Edvinsson, L},
abstractNote = {In patients undergoing aorto-coronary by-pass surgery, we found a 26% arterial-venous difference of immunoreactive {gamma}{sub 2}-melanocytostimulating hormone (MSH), a proopiomelanocortin (POMC) derived peptide known to possess profound hemodynamic effects. These results prompted an investigation of the presence of {gamma}{sub 2}-MSH in the human heart. Using a two-step extraction procedure, regions of human hearts were examined by sensitive and specific radioimmunoassays to determine their {gamma}{sub 2}-MSH content. Mean ({plus minus} SEM) concentrations of 0.14 {plus minus} 0.023 pmol/g and 0.12 {plus minus} 0.017 were found in right atrium and right ventricle, respectively. High performance liquid chromatography indicated that 80-90 % of the total immunoreactivity eluted in a single sharp peak in a position identical to that of synthetic {gamma}{sub 2}-MSH.},
doi = {10.1016/0024-3205(89)90171-9},
url = {https://www.osti.gov/biblio/7195035},
journal = {Life Sciences; (USA)},
issn = {0024-3205},
number = ,
volume = 45:9,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}