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Title: Plasma membrane reorganization induced by chemical transformation in cultura

Induction of increased rigidity in the plasma membrane paralleling properties associated with a transformed state was suggested by two experiments. Fluorescence recovery after photobleaching (FRAP) indicated the induction of an environment in the plasma membrane where the synthetic fluorescent phospholipid collarein was immobile on the FRAP timescale. The other technique revealed the binding of epidermal growth factor (EGF) to a cryptic class of receptors which become accessible upon chemical transformation. These two lines of evidence are consistent with a reorganization of the plasma membrane induced by tumor promoters. 110 references, 38 figures, 4 tables.
Authors:
Publication Date:
OSTI Identifier:
7192531
Report Number(s):
LBL-17712
ON: DE84012311
DOE Contract Number:
AC03-76SF00098
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions are illegible in microfiche products. Thesis
Research Org:
Lawrence Berkeley Lab., CA (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL MEMBRANES; BIOCHEMISTRY; BIOPHYSICS; ONCOGENIC TRANSFORMATIONS; CELL CULTURES; LIPIDS; LIPOSOMES; MASS SPECTROSCOPY; NUCLEAR MAGNETIC RESONANCE; PHORBOL ESTERS; CARCINOGENS; CELL CONSTITUENTS; CHEMISTRY; ESTERS; MAGNETIC RESONANCE; MEMBRANES; ORGANIC COMPOUNDS; ORGANOIDS; RESONANCE; SPECTROSCOPY 550200* -- Biochemistry; 560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)