Characterization of a rapid, blue light-mediated change in detectable phosphorylation of a plasma membrane protein from etiolated pea (Pisum sativum L. ) seedlings

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.1.179· OSTI ID:7192039

Short, T W; Briggs, W R ^[1]

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1. Stanford Univ., CA (USA)

When crude microsomal membranes from apical stem segments of etiolated Pisum sativum L. cv Alaska are mixed in vitro with {gamma}-({sup 32}P)ATP, a phosphorylated band of apparent molecular mass 120 kilodaltons can be detected on autoradiographs of sodium dodecyl sulfate electrophoresis gels. If the stem sections are exposed to blue light immediately prior to membrane isolation, this band is not evident. Comparisons of the kinetics, tissue distribution, and dark recovery of the phosphorylation response with those published for blue light mediated phototropism or rapid growth inhibition indicate that the phosphorylation could be linked to one or both of the reactions described. However, the fluence-response relationships for the change in detectable phosphorylation match quite closely those reported for phototropism but not those for growth inhibition. Blue light has also been found to regulate the capacity for in vitro phosphorylation of a second protein. It has an apparent molecular mass of 84 kilodaltons and is localized primarily in basal stem sections.

• OSTI ID: 7192039
• Journal Information: Plant Physiology; (USA), Vol. 92:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PISUM
PLANT GROWTH
VISIBLE RADIATION
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
CELL MEMBRANES
PHOSPHORUS 32
PHOSPHORYLATION
PLANT STEMS
PROTEINS
TISSUE DISTRIBUTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTROMAGNETIC RADIATION
GROWTH
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIATIONS
RADIOISOTOPES
551001* - Physiological Systems- Tracer Techniques