Comparative biochemistry of betaine biosynthesis and accumulation in diverse dicot families

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:7190501

McCue, K F; Weretilnyk, E A; Bednarek, S Y; Hanson, A D ^[1]

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1. Michigan State Univ., East Lansing (USA)

Salt stress elicits betaine accumulation to high levels in species from several diverse dicot families (Chenopodiaceae, Amaranthaceae, Convolvulaceae, Solanaceae, and Asteraceae). FAM-MS studies with deuterated precursors showed that species from all these families synthesize betaine from choline. Enzyme assays and immunotitration data showed that all accumulating species contained betaine aldehyde dehydrogenase (BADH) enzyme activity recognized by antibodies raised against purified BADH isolated from Spinacia oleracea. Immunoblotting indicated that the BADH monomer was in all cases of Mr {approx} 63,000. The similarity of BADH in the different species is consistent with a single evolutionary origin for the betaine pathway. This was supported by the presence in immunoblots of a cross-reacting band at Mr {approx} 63,000 in Magnolia x Soulangiana, a primitive angiosperm.

• OSTI ID: 7190501
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BETAINE
BIOSYNTHESIS
PLANTS
PHYSIOLOGY
BIOLOGICAL PATHWAYS
SODIUM CHLORIDES
TOXICITY
ALKALI METAL COMPOUNDS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
DRUGS
HALIDES
HALOGEN COMPOUNDS
LIPOTROPIC FACTORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
QUATERNARY COMPOUNDS
SODIUM COMPOUNDS
SYNTHESIS
551000* - Physiological Systems