Specific binding and uptake of apolipoprotein E-free high density lipoproteins (apo E-free HDL) by cultured liver parenchymal cells of copper-deficient rats

Journal Article · Thu Feb 09 00:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:7189704

Zhang, J; Carr, T P; Lei, K Y ^[1]

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1. Univ. of Arizona, Tucson (USA)

Weanling male Sprague-Dawley rats were assigned to two dietary treatments; copper-deficient (CD, 1.0 ppm) and adequate (CA, 6.7 ppm). After 8 weeks, plasma apo E-free HDL were isolated by a combination of ultracentrifugation, gel filtration, and heparin-Sepharose affinity chromatography. Liver parenchymal cells obtained by collagenase perfusion and Percoll gradient centrifugation were cultured for 16 hr. Cells were then incubated with {sup 125}I-apo E-free HDL, either from the same treatment group or in a crossover design, in order to establish if treatment differences were associated with cells and/or lipoproteins. Cells were incubate with apo E-free HDL for 2, 6, 12, and 24 hr in the presence or absence of excess unlabeled apo E-free HDL. Binding studies at 4{degree}C indicated a small but significant increases in specific binding in CD cells regardless of the source of apo E-free HDL. In cell association studies at 37{degree}C, CD cells demonstrated similar marked increases of apo E-free HDL uptake from both treatment groups. The increase in uptake may be associated with an increased expression of HDL binding sites in CD cells. These findings are consistent with recent in vivo studies which indicate plasma clearance and tissue uptake of total HDL are increased in copper-deficient rats.

• OSTI ID: 7189704
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Vol. 3:3; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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BIOLOGICAL EFFECTS
LIPOPROTEINS
BIOCHEMICAL REACTION KINETICS
APOLIPOPROTEINS
CELL CULTURES
IODINE 125
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IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
MAMMALS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
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