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Title: Activation of brown adipose tissue mitochondrial GDP binding sites

The primary function of brown adipose tissue (BAT) is heat production. This ability is attributed to the existence of a unique inner mitochondrial membrane protein termed the uncoupling protein or thermogenin. This protein is permeable to H+ and thus allows respiration (and therefore thermogenesis) to proceed at a rapid rate, independent of ADP phosphorylation. Proton conductance can be inhibited by the binding of purine nucleotides to the uncoupling protein. The binding of (/sup 3/H)-GDP to BAT mitochondria is frequently used as a measure of BAT thermogenic activity. Rats fed a diet that was low but adequate in protein exhibited a decrease in feed efficiency. In addition, BAT thermogenesis was activated as indicated by an elevation in the level of GDP binding to BAT mitochondria. This phenomena occurred in older rats and persisted over time.
Authors:
Publication Date:
OSTI Identifier:
7188086
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Univ. of Wisconsin,Madison, WI
Research Org:
Wisconsin Univ., Madison (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADIPOSE TISSUE; BIOLOGICAL FUNCTIONS; NUCLEOTIDES; BIOCHEMICAL REACTION KINETICS; RECEPTORS; MEMBRANE PROTEINS; MITOCHONDRIA; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL TISSUES; BODY; CELL CONSTITUENTS; CONNECTIVE TISSUE; FUNCTIONS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; ORGANOIDS; PROTEINS; REACTION KINETICS; TISSUES 550201* -- Biochemistry-- Tracer Techniques