Incorporation of bacterial peptidoglycan constituents into macrophage lipids during phagocytosis. [Bacillus subtillis]
Bacillus subtilis radiolabeled cell walls were incubated with the macrophage cell line RAW264 in order to determine whether a peptidoglycan fragment were subsequently maintained on a macrophage lipid. Specifically, cell walls were radiolabeled in their glucosamine, muramic acid and alanine residues with D-(1-/sup 3/H) glucosamine and L(U-/sup 14/C)alanine. Following encounter with these radiolabeled cell walls, macrophages were collected and subjected to lipid extraction procedures. Further fractionation produced a phosphatidylethanolamine co-migrating lipid which upon hydrolysis and amino acid analysis revealed radiolabeled muramic acid, glucosamine, and alanine residues. These residues were shown to form a common fragment since the aqueous soluble material obtained after saponification of the crude lipid extract eluted as a single peak following gel permeation chromatography. Saponification destroyed the TLC mobility of the lipid showing that the fragment was covalently attached to the lipid.
- Research Organization:
- Minnesota Univ., Minneapolis (USA)
- OSTI ID:
- 7188072
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHAGOCYTOSIS
BIOCHEMICAL REACTION KINETICS
ALANINES
BACILLUS SUBTILIS
CARBON 14 COMPOUNDS
CELL WALL
FRACTIONATION
GLUCOSAMINE
LIGANDS
LIPIDS
MACROPHAGES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL CELLS
BACILLUS
BACTERIA
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques