Chlorate: a reversible inhibitor of proteoglycan sulfation

Journal Article · Fri Jul 15 00:00:00 EDT 1988 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(88)90694-8· OSTI ID:7187848

Humphries, D E; Silbert, J E

Bovine aorta endothelial cells were cultured in medium containing (/sup 3/H)glucosamine, (/sup 35/S)sulfate, and various concentrations of chlorate. Cell growth was not affected by 10 mM chlorate, while 30 mM chlorate had a slight inhibitory effect. Chlorate concentrations greater than 10 mM resulted in significant undersulfation of chondroitin. With 30 mM chlorate, sulfation of chondroitin was reduced to 10% and heparan to 35% of controls, but (/sup 3/H)glucosamine incorporation on a per cell basis did not appear to be inhibited. Removal of chlorate from the culture medium of cells resulted in the rapid resumption of sulfation.

• Research Organization: Veterans Administration Outpatient Clinic, Boston, MA (USA)
• OSTI ID: 7187848
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 154:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLORATES
BIOCHEMICAL REACTION KINETICS
CHONDROITIN
METABOLISM
ANIMAL CELLS
AORTA
CATTLE
CELL CULTURES
ENDOTHELIUM
GLUCOSAMINE
INHIBITION
SULFATES
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL TISSUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
HALOGEN COMPOUNDS
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques