Applications of surface-enhanced Raman spectroscopy for the study of indicators of exposure to selected genotoxins

Miscellaneous · Wed Jan 01 00:00:00 EST 1992

OSTI ID:7171332

Helmenstine, A M

Surface-enhanced Raman scattering spectroscopy (SERS) was used to detect and analyze certain genotoxic chemicals, their metabolites, and selected deoxyribonucleic acid/ribonucleic acid (DNA/RNA) adducts that may be produced from exposure to specific toxins. Substances examined included nucleic acid (purine/pyrimidine) etheno adducts of adenine and cytosine and carcinogens benzo[a]pyrene and aflatoxins. SERS was shown to be an effective tool for sensitive, selective detection and analysis of these genotoxins and related substances. Detection was accomplished even at such low concentrations as might be present in biological systems, and in minute volumes of approximately one microliter per sample. Most substances were found to possess unique spectral signatures and could be distinguished from other chemicals in this study. The sample molecules were also analyzed in body fluids (e.g., plasma, serum, and urine) and adducted to DNA. In this study SERS was demonstrated to open a new dimension in chemical analysis not previously available by utilizing other spectroscopies or methods of chemical detection. Special attention was given to the various factors affecting the quality and reproducibility of a SERS spectrum, emphasizing the importance of optimization of Raman cross-scattering enhancement. Methods for adapting SERS to deal with specific research problems and for expanding the capabilities of SERS to address the health effects from human exposure to toxic pollutants were addressed in the concluding remarks section of this manuscript.

• Research Organization: Tennessee Univ., Knoxville, TN (United States)
• OSTI ID: 7171332
• Resource Relation: Other Information: Thesis (Ph.D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
74 ATOMIC AND MOLECULAR PHYSICS
BIOLOGICAL MARKERS
RAMAN SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
CARCINOGENS
DNA ADDUCTS
AFLATOXINS
BENZOPYRENE
BODY FLUIDS
HEALTH HAZARDS
METABOLITES
SENSITIVITY
TOXINS
ADDUCTS
ANTIGENS
AROMATICS
BIOLOGICAL MATERIALS
CONDENSED AROMATICS
HAZARDOUS MATERIALS
HAZARDS
HYDROCARBONS
MATERIALS
MYCOTOXINS
ORGANIC COMPOUNDS
SPECTRA
TOXIC MATERIALS
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