Cellular and molecular aspects of early bone development in the chick embryonic tibia

Weitzhandler, M

Title: Cellular and molecular aspects of early bone development in the chick embryonic tibia

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Abstract

Mid-diaphyseal periosteal collars and the corresponding cartilage core were micro-dissected free from chick tibias and separately digested with a trypsin-collagenase enzyme mixture. The released cell populations were cultivated in vitro and characterized by morphological analysis, histochemical localization, of alkaline phosphatase, alizarin red S staining for mineral deposition, growth rate ((/sup 3/H)thymidine incorporation) and proteoglycan content. Results of these studies showed that periosteal collar cell cultures form nodule-like structures that stain positively with alkaline phosphatase and alizarin red S. Light and electron microscopic observation revealed cell and matrix morphologies similar to that of intact periosteum. The nodules were composed of plump cell types embedded within a mineralized matrix surrounded by a fibroblastic cell layer. Core cartilage cell cultures displayed typical characteristics of the hypertrophic state in their visual appearance and proteoglycan composition. The formation of osseous-like structures in periosteal collar cell cultures but not in core chondrocyte cell cultures demonstrates the relatively autonomous nature of periosteal ossification.

Authors:: Weitzhandler, M

Publication Date:: Tue Jan 01 00:00:00 EST 1985

Research Org.:: Case Western Reserve Univ., Cleveland, OH (USA)

OSTI Identifier:: 7170342

Resource Type:: Thesis/Dissertation

Resource Relation:: Other Information: Thesis (Ph. D.)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; BONE CELLS; GROWTH; MORPHOLOGY; EMBRYOS; MINERALIZATION; ALIZARIN; ALKALINE PHOSPHATASE; CARTILAGE; CELL CULTURES; CHICKENS; DIGESTION; ELECTRON MICROSCOPY; EXPERIMENTAL DATA; FIBROBLASTS; IN VITRO; THYMIDINE; TIBIA; TRACER TECHNIQUES; TRITIUM COMPOUNDS; TRYPSIN; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTHRAQUINONES; AROMATICS; AZINES; BIRDS; BODY; CONNECTIVE TISSUE; CONNECTIVE TISSUE CELLS; DATA; DYES; ENZYMES; ESTERASES; FOWL; HETEROCYCLIC COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; INFORMATION; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROSCOPY; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; PEPTIDE HYDROLASES; PHOSPHATASES; PYRIMIDINES; QUINONES; REAGENTS; RIBOSIDES; SERINE PROTEINASES; SKELETON; SOMATIC CELLS; TISSUES; VERTEBRATES; 550801* - Morphology- Tracer Techniques

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                    Weitzhandler, M. Cellular and molecular aspects of early bone development in the chick embryonic tibia.  United States: N. p., 1985. 
        Web.

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                    Weitzhandler, M. Cellular and molecular aspects of early bone development in the chick embryonic tibia.  United States.

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                    Weitzhandler, M. 1985.  
        "Cellular and molecular aspects of early bone development in the chick embryonic tibia".  United States.

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@article{osti_7170342,

  title        = {Cellular and molecular aspects of early bone development in the chick embryonic tibia},

  author       = {Weitzhandler, M},

  abstractNote = {Mid-diaphyseal periosteal collars and the corresponding cartilage core were micro-dissected free from chick tibias and separately digested with a trypsin-collagenase enzyme mixture. The released cell populations were cultivated in vitro and characterized by morphological analysis, histochemical localization, of alkaline phosphatase, alizarin red S staining for mineral deposition, growth rate ((/sup 3/H)thymidine incorporation) and proteoglycan content. Results of these studies showed that periosteal collar cell cultures form nodule-like structures that stain positively with alkaline phosphatase and alizarin red S. Light and electron microscopic observation revealed cell and matrix morphologies similar to that of intact periosteum. The nodules were composed of plump cell types embedded within a mineralized matrix surrounded by a fibroblastic cell layer. Core cartilage cell cultures displayed typical characteristics of the hypertrophic state in their visual appearance and proteoglycan composition. The formation of osseous-like structures in periosteal collar cell cultures but not in core chondrocyte cell cultures demonstrates the relatively autonomous nature of periosteal ossification.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/7170342},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 01 00:00:00 EST 1985},

  month        = {Tue Jan 01 00:00:00 EST 1985}

}

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