Specific binding of sup 125 I-rErythropoietin to Friend polycythemia virus-transformed erythroleukemia cells purified by centrifugal elutriation

Journal Article · Mon Jan 01 00:00:00 EST 1990 · American Journal of Hypertension; (USA)

DOI:https://doi.org/10.1002/stem.5530080106· OSTI ID:7168192

Correa, P N; Bard, V; Axelrad, A A ^[1]

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1. Univ. of Toronto (Canada)

We have used countercurrent centrifugal elutriation (CCE) to determine the distribution of cells with respect to cell volume and buoyant density for an erythroleukemia cell line (JG6) transformed by the polycythemia strain of Friend virus (FV-P), and to determine the effect of inducing the cells to differentiate with dimethylsulfoxide (DMSO) on this distribution. CCE made it possible to obtain suspensions of modal JG6 populations virtually free of dead cells and uniform with respect to volume and buoyant density. These modal populations were assayed for specific binding of erythropoietin (Epo). Between 500 and 550 Epo receptors per cell were detected. These belonged to a single class having a dissociation constant of 0.36 nM. DMSO induction of differentiation of the JG6 cells had no effect on the number of Epo receptors expressed.

• OSTI ID: 7168192
• Journal Information: American Journal of Hypertension; (USA), Vol. 8:1; ISSN 0737-1454
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ERYTHROPOIETIN
BIOCHEMICAL REACTION KINETICS
TUMOR CELLS
CELL DIFFERENTIATION
CELL TRANSFORMATIONS
CENTRIFUGATION
DMSO
IODINE 125
LEUKEMIA
RECEPTORS
TRACER TECHNIQUES
VIRUSES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SULFOXIDES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques