Specific estrogen-binding protein of rat liver and sex steroid metabolism
Model experiments were conducted to study the effect of a highly purified preparation of specific estrogen-binding protein (SEBP) on the intensity of estradiol and testosterone metabolism under the influence of enzymes in liver homogenate from female rats, not containing SEBP. The liver of mature female rats was homogenized in two volumes of 50 mM Tris-HCl buffer, pH 7.5, containing 600 mg% of glucose. The tritium-steroid was preincubated for 15 min at 0-4 C with 0-4 microg of the preparation of SEBP (200 microl). A standard preparation of partially purified SEBP was obtained from liver cystosol of mature male rats; affinity chromatography on estradiolagarose was used. It is shown that SEBP can really take part in regulation of the dynamics of sex steroids in the liver. E/sub 1/ did not affect the metabolic rate of H 3-E/sub 2/ by liver homogenate from females, but caused marked acceleration of H 3-E/sub 2/ metabolism by male liver homogenate.
- Research Organization:
- Laboratory of Endocrinology, Faculty of Biology, M.V. Lomonosov Moscow University
- OSTI ID:
- 7166174
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:8
- Country of Publication:
- United States
- Language:
- English
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ESTRADIOL
METABOLISM
LIVER CELLS
TRACER TECHNIQUES
PROTEINS
BIOLOGICAL EFFECTS
TESTOSTERONE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
FEMALES
HYDROCHLORIC ACID
LIVER
RATS
RETENTION
SCINTILLATION COUNTING
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
BODY
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
ESTRANES
ESTROGENS
FUNCTIONS
GLANDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INORGANIC ACIDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques