Isolation from bovine brain of substances inhibiting specific binding of imipramine and serotonin uptake

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:7166169

Mukhin, A G; Kladnitskii, A V; Kovaleva, E S; Kudryakova, T B

The authors search for endogenous ligands of the ''imipramine receptor'' in brain tissue. Binding of tritium-imipramine with the fraction of unpruified bovine brain synaptic membranes was carried out by the method of Raisman et'al. Uptake of tritium-serotonin by synaptosomes of rat cerebral cortex was estimated. The results do not give a final anser to the question of the existence of an endogenous ligand of the ''imipramine receptor'' but they can serve as the basis for research aimed at purifying the active fractions already obtained and identifying the compounds containined in them.

• Research Organization: Laboratory of Biochemistry, AllUnion Mental Health Research Center, Moscow
• OSTI ID: 7166169
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CATTLE
BRAIN
IMIPRAMINE
RADIORECEPTOR ASSAY
UPTAKE
SEROTONIN
BIOLOGICAL PATHWAYS
CELL MEMBRANES
INHIBITION
LIGANDS
NERVE CELLS
RATS
RECEPTORS
RETENTION
SAMPLE PREPARATION
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
ANTIDEPRESSANTS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DOMESTIC ANIMALS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PSYCHOTROPIC DRUGS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
RUMINANTS
SOMATIC CELLS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550501 - Metabolism- Tracer Techniques