Removal of drugs from the circulation using immobilized monoclonal antibodies

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7164302

Brizgys, M V

High-affinity monoclonal antidigoxin antibodies (dig-Ab) were immobilized to a pellicular microbead and characterized in terms of antibody affinity, specificity for other glycosides, and binding capacity. Determination of digoxin binding revealed that the binding capacity decreased to 25% of theoretical capacity. Attempts to improve the binding capacity were ineffective. A guinea pig animal model was developed to determine the efficacy of removing digoxin in vivo from the circulation using an antibody column. Male guinea pigs were hemoperfused with either a dig-Ab or bovine Y-globulin control column 16 h after a single i.v. injection of digoxin. Pre- and postcolumn plasma concentrations were obtained to evaluate the extraction efficiency. Hemoperfusion continued for 3 h at flow rates of 1.0-2.0 mL/min. Bound digoxin was eluted as described earlier and concentrations determined by (/sup 125/I) digoxin RIA. Amounts of digoxin removed represented less than 1% of the total body content. After several studies with the same column, the dig-Ab had lost most of its activity. A freshly prepared dig-Ab column removed approximately 20% of the total body content. Most of the measured constituents of the blood were unaffected by the procedure.

• Research Organization: Utah Univ., Salt Lake City (USA)
• OSTI ID: 7164302
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIGOXIN
BIOCHEMICAL REACTION KINETICS
RADIOIMMUNOASSAY
REMOVAL
MONOCLONAL ANTIBODIES
CHEMICAL COMPOSITION
SPECIFICITY
AFFINITY
BIOLOGICAL MODELS
GUINEA PIGS
IODINE 125
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DIGITALIS GLYCOSIDES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOSIDES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques