Complex mixture analysis using protein expression as a qualitative and quantitative tool
- Univ. of Maryland, Baltimore, MD (United States). Dept. of Biological Sciences
- Procter and Gamble Co., Cincinnati, OH (United States). Paper Products Division
Some proteins in organisms exposed to chemicals in stressful amounts or toxic concentrations show increased expression; others show decreased expression. These inducible and repressible proteins together potentially provide qualitative and quantitative diagnoses of components in complex mixtures of chemicals. The authors examined sets of proteins synthesized by Daphnia magna after exposure to mixtures of a cationic polyamide epichlorhydrin adduct (Kymene) and a combined assortment of water-extractable substances from chemi-thermal-mechanical pulp (CTMP) in lab water. Proteins were identified, after extracting from Daphnia magna, by gel filtration and silver staining, or by radiolabeling and then gel separation. Patterns of proteins induced by Kymene[reg sign] and by CTMP extracts were distinguishable in lab water, but there was interaction between them. The method of identifying and quantifying Kymene, however, was successful using lab simulations of mixtures. The method was tested using wastewater samples from a paper manufacturing plant. Kymene could be detected against variable levels and types of additional substances. But, again, there was interference, perhaps due to Kymene binding to other anionic polymers sometimes present in the samples. Interpretation from analyses of protein expression were consistent with results from sublethal Ceriodaphnia dubia assays.
- OSTI ID:
- 7162047
- Journal Information:
- Environmental Toxicology and Chemistry; (United States), Vol. 13:7; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DAPHNIA
SENSITIVITY
PROTEINS
BIOSYNTHESIS
WASTE WATER
CHEMICAL ANALYSIS
METABOLISM
CHEMICAL EFFLUENTS
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
BRANCHIOPODS
CHEMICAL WASTES
CRUSTACEANS
HYDROGEN COMPOUNDS
INVERTEBRATES
LIQUID WASTES
NONRADIOACTIVE WASTES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
SYNTHESIS
WASTES
WATER
560300* - Chemicals Metabolism & Toxicology