Recovery from severe hematopoietic suppression using recombinant human granulocyte-macrophage colony-stimulating factor
The ability of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) to enhance recovery of a radiation-suppressed hematopoietic system was evaluated in a nonuniform radiation exposure model using the rhesus monkey. Recombinant human GM-CSF treatment for 7 days after a lethal, nonuniform radiation exposure of 800 cGy was sufficient to enhance hematopoietic reconstitution, leading to an earlier recovery. Monkeys were treated with 72,000 U/kg/day of rhGM-CSF delivered continuously through an Alzet miniosmotic pump implanted subcutaneously on day 3. Treated monkeys demonstrated effective granulocyte and platelet levels in the peripheral blood, 4 and 7 days earlier, respectively, than control monkeys. Granulocyte-macrophage colony-forming unit (CFU-GM) activity in the bone marrow was monitored to evaluate the effect of rhGM-CSF on marrow recovery. Treatment with rhGM-CSF led to an early recovery of CFU-GM activity suggesting that rhGM-CSF acted on an earlier stem cell population to generate CFU-GM. Thus, the effect of rhGM-CSF on hematopoietic regeneration, granulocyte recovery, and platelet recovery are evaluated in this paper.
- Research Organization:
- Naval Medical Research Institute, Bethesda, MD (USA)
- OSTI ID:
- 7138725
- Journal Information:
- Exp. Hematol.; (United States), Vol. 16:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROWTH FACTORS
RADIOSENSITIVITY EFFECTS
HEMATOPOIETIC SYSTEM
BIOLOGICAL RECOVERY
BLOOD PLATELETS
BONE MARROW
COLONY FORMATION
LETHAL IRRADIATION
LEUKOCYTES
MACROPHAGES
MONKEYS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
IRRADIATION
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PRIMATES
PROTEINS
RECOVERY
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals