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Title: Effect of placental factors on growth and function of the human fetal adrenal in vitro

Conditioned medium from human placental monolayer cultures (PM) had a marked stimulatory effect on proliferation (3H-thymidine uptake) of human fetal zone adrenal cells in primary monolayer culture, even in the absence of serum. Epidermal growth factor (EGF) and fibroblast growth factor (FGF) also significantly stimulated fetal adrenal cell growth. However, the effects of PM differed from those of EGF and FGF in several respects: (1) maximal response to PM was 2-5 times greater; (2) mitogenic effects of EGF and FGF were suppressed by adrenocorticotropic hormone (ACTH), whereas that of 50% PM was not; (3) PM inhibited ACTH-stimulated steroidogenesis (dehydroepiandrosterone sulfate and cortisol), but EGF and FGF did not. Preliminary characterization studies have indicated that approximately half of the placental growth-promoting activity is heat resistant and sensitive to bacterial proteases, and that 50-60% of the activity is lost after dialysis with membranes having a molecular weight cutoff of 3500. These findings suggest a role for the placenta in the growth and differentiated function of the human fetal adrenal gland.
Authors:
; ; ; ; ; ;  [1]
  1. (McGill Univ.-Montreal Children's Hospital Research Institute, Quebec (Canada))
Publication Date:
OSTI Identifier:
7138024
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biology of Reproduction; (USA); Journal Volume: 41:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADRENAL GLANDS; BIOLOGICAL FUNCTIONS; ANIMAL CELLS; CELL PROLIFERATION; GROWTH FACTORS; BIOCHEMICAL REACTION KINETICS; ACTH; CELL CULTURES; CULTURE MEDIA; FETUSES; MAN; PREGNANCY; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AZINES; BODY; ENDOCRINE GLANDS; FUNCTIONS; GLANDS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; MITOGENS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; PRIMATES; PROTEINS; PYRIMIDINES; REACTION KINETICS; RIBOSIDES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques