Structural features of class I H-2 antigens revealed by anti-peptide antibodies specific for intracytoplasmic determinants

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7135124

Barber, B H; Smith, M H

Rabbit antisera specific for synthetic peptides corresponding to each of the three intracytoplasmic exons (i.e. exons 6, 7 and 8) of the H-2K/sup b/ gene have been prepared and characterized in terms of their reactivity with class I H-2 molecules. Using lentil lectin-purified glycoproteins isolated from /sup 125/I-surface-labelled EL-4 (H-2/sup b/) cells, it has been possible to demonstrate that after clearance of the glycoprotein preparation with either anti-mouse ..beta../sub 2/-microglobulin, or alloantisera directed against H-2K/sup b/ and D/sup b/, there remains a non-..beta../sub 2/-microglobulin-associated (i.e. free) class I heavy chain which can be immunoprecipitated by anti-peptide 8 antisera. This free class I heavy chain has the same 2D gel spot pattern as heavy chains from dimeric H-2K/sup b/, suggesting that conformational alterations resulting from the dissociation of ..beta../sub 2/-microglobulin, rather than chemical changes, are responsible for the loss of the alloantigenic determinants. Evidence has also been obtained indicating that the reaction of dimeric class I molecules with anti-peptide 6 induces the dissociation of ..beta../sub 2/-microglobulin, suggesting the potential for significant transmembrane conformational perturbations of the external domains as a result of interactions with the intracytoplasmic region.

• Research Organization: Univ. of Toronto, Ontario
• OSTI ID: 7135124
• Report Number(s): CONF-8604222-; TRN: 87-005728
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
MOLECULAR STRUCTURE
ANIMAL CELLS
ANTIGEN-ANTIBODY REACTIONS
GLUCOPROTEINS
IODINE 125
MOLECULES
RABBITS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques