The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.4.1432· OSTI ID:7131057

Boeke, J D; Eichinger, D; Castrillon, D; Fink, G R

Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.

• Research Organization: Dept. of Molecular Biology and Genetics, Johns Hopkins Univ. School of Medicine, 725 N. Wolfe Street, Baltimore, MD (US)
• OSTI ID: 7131057
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SACCHAROMYCES CEREVISIAE
CHROMOSOMAL ABERRATIONS
DNA
GENETIC MAPPING
MUTATIONS
CHROMOSOME LOSSES
RECOMBINANT DNA
TRANSPOSONS
FUNGI
LOSSES
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
YEASTS
550700* - Microbiology
550400 - Genetics