A photoactivatable probe for the Na+/H+ exchanger cross-links a 66-kDa renal brush border membrane protein
- Washington Univ. School of Medicine, St. Louis, MO (USA)
Earlier studies on LLC-PK1 cells have demonstrated two pharmacologically distinct Na+/H+ exchangers in renal epithelia. In addition, the cDNA clone for the human Na+/H+ antiporter which is growth factor activatable has been isolated and expressed. We report here the synthesis of an amiloride analogue that can be photoactivated and labeled with 125I. This analogue covalently cross-links a 66-kDa protein of bovine renal brush border membranes. A rabbit polyclonal antibody that was directed against a 20-amino acid peptide of the cytoplasmic domain of its human Na+/H+ antiporter also gives a positive Western against 66-kDa protein of bovine brush border membranes. Thus, the photoactive probe may be helpful in the isolation and purification of the brush border Na+/H+ exchanger.
- OSTI ID:
- 7118073
- Journal Information:
- Journal of Biological Chemistry; (USA), Vol. 265:10; ISSN 0021-9258
- Country of Publication:
- United States
- Language:
- English
Similar Records
Photoaffinity labeling by ( sup 3 H)-N5-methyl-N5-isobutylamiloride of proteins which cofractionate with Na+/H+ antiport activity
Molecular size of the Na+-H+ antiport in renal brush border membranes, as estimated by radiation inactivation
Related Subjects
DIURETICS
BIOLOGICAL FUNCTIONS
MEMBRANE PROTEINS
CROSS-LINKING
ANTIBODIES
CATTLE
CHEMICAL PREPARATION
DOGS
IODINE 125
KIDNEYS
MASS SPECTRA
MOLECULAR WEIGHT
PROTEIN STRUCTURE
RABBITS
REAGENTS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RUMINANTS
SPECTRA
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques