Prostaglandins as mediators of acidification in the urinary bladder of Bufo marinus

Journal Article · Tue May 01 00:00:00 EDT 1990 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

DOI:https://doi.org/10.3181/00379727-194-43046· OSTI ID:7117951

Frazier, L W; Yorio, T ^[1]

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1. Baylor College of Dentistry, Dallas, TX (USA)

Experiments were performed to determine whether prostaglandins (PG) play a role in H+ and NH4+ excretion in the urinary bladder of Bufo marinus. Ten paired hemibladders from normal toads were mounted in chambers. One was control and the other hemibladder received PGE2 in the serosal medium (10(-5) M). H+ excretion was measured by change in pH in the mucosal fluid and reported in units of nmol (100 mg tissue)-1 (min)-1. NH4+ excretion was measured colorimetrically and reported in the same units. The control group H+ excretion was 8.4 +/- 1.67, while the experimental group was 16.3 +/- 2.64 (P less than 0.01). The NH4+ excretion in the experimental and control group was not significantly different. Bladders from toads in a 48-hr NH4+Cl acidosis (metabolic) did not demonstrate this response to PGE2 (P greater than 0.30). Toads were put in metabolic acidosis by gavaging with 10 ml of 120 mM NH4+Cl 3 x day for 2 days. In another experiment, we measured levels of PG in bladders from control (N) and animals placed in metabolic acidosis (MA). Bladders were removed from the respective toad, homogenized, extracted, and PG separated using high-pressure liquid chromatography and quantified against PG standards. The results are reported in ng (mg tissue)-1. PGE2 fraction in N was 1.09 +/- 0.14 and in MA was 3.21 +/- 0.63 (P less than 0.01). PGF1 alpha, F2 alpha and I2 were not significantly different in N and MA toads. Bladders were also removed from N and MA toads, and incubated in Ringer's solution containing (3H)arachidonic acid (0.2 microCi/ml) at 25 degrees C for 2 hr. Bladders were then extracted for PG and the extracts separated by thin layer chromatography. PG were identified using standards and autoradiography, scraped from plates, and counted in a scintillation detector. The results are reported in cpm/mg tissue x hr +/- SEM.

• OSTI ID: 7117951
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Vol. 194:1; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMMONIUM COMPOUNDS
EXCRETION
BLADDER
ACIDIFICATION
PROSTAGLANDINS
BIOCHEMICAL REACTION KINETICS
PROTONS
ARACHIDONIC ACID
AUTORADIOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
FROGS
IN VITRO
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
PH VALUE
SCINTILLATION COUNTING
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BARYONS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CLEARANCE
COUNTING TECHNIQUES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
KINETICS
MONOCARBOXYLIC ACIDS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SEPARATION PROCESSES
URINARY TRACT
VERTEBRATES
550201* - Biochemistry- Tracer Techniques