Use of a genetically engineered Escherichia coli strain to produce 1,2-dihydroxy-4 prime -chlorobiphenyl

Journal Article · Wed Apr 01 00:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7114693

Khan, A A; Walia, S K ^[1]

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1. Oakland Univ., Rochester, MI (United States)

Genetically engineered kanamycin-resistant Escherichia coli HB101 containing the mutant chimeric plasmid pAW6194-T17 specifying biphenyl dioxygenase and dihydrodiol dehydrogenase and lacking the ability to produce active 3-phenylcatechol dioxygenase was used to produce 1,2-dihydroxy-4{prime}-chlorobiphenyl (DHCB) from 4-chlorobiphenyl produced 110 {mu}M DHCB. The K{sub m} for 4-chlorobiphenyl was 3.3 mM. Biotransformation of DHCB from 4-chlorobiphenyl was maximum when cells (2.5 mg of protein per ml) were incubated with shaking (150 rpm) at pH 7.0 and 30C for 6 h. The enzymatically produced DHCB was a suitable substrate for assaying 3-phenylcatechol dioxygenase activity. Biologically produced DHCB showed UV and mass spectra similar to those of chemically synthesized DHCB. The bioconversion rate of ortho-substituted chlorobiphenyl was slower than that of the para- or meta-substituted chlorobiphenyl.

• OSTI ID: 7114693
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 58:4; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLORINATED AROMATIC HYDROCARBONS
BIOSYNTHESIS
ESCHERICHIA COLI
GENETIC ENGINEERING
METABOLITES
OXYGENASES
PLASMIDS
SUBSTRATES
USES
AROMATICS
BACTERIA
BIOTECHNOLOGY
CELL CONSTITUENTS
ENZYMES
HALOGENATED AROMATIC HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PROTEINS
SYNTHESIS
550200* - Biochemistry