N-acetylneuraminlactose sulfate in milk and its role in the synthesis of glycosaminoglycans during development
Mammals in early life are incapable of synthesizing inorganic sulfate, an important constituent of connective tissue glycosaminoglycans (GAGs). Studies have shown that (/sup 35/S)Na/sub 2/SO/sub 4/ injected into lactating rats is secreted into the milk as (/sup 35/S) N-acetylneuraminlactose sulfate (NLS); this compound accounts for greater than or equal to 95% of the radioactivity found in milk. They have studied the metabolic fate of the sulfate moiety of NLS in the newborn rat. Lactating rat dams were injected with (/sup 35/S) Na/sub 2/SO/sub 4/ at various times postpartum and pups were allowed to suckle for 48 hrs before sacrifice. Pups of varying ages (3 d. - 19 d.) were sacrificed and the GAGs were prepared from 9 different organs and tissues. Pups of all ages showed appreciable radiosulfate incorporated into all of the tissues and organs examined. In young pups (3 and 5 d old) a high of 75% of the total (/sup 35/S) sulfate in the homogenate was found in the GAGs of bone, and a low of 27% in the GAGs of liver. Analysis of the composition of the GAGs by enzymatic degradation showed that bone GAGs consisted entirely of chondroitin 4/6 sulfate (C4/6S) while liver GAGs contained 50% C4/6S, 10% dermatan sulfate (DS), and 40% chondroitinase resistant material (probably heparan sulfate). These data show that sulfate in the form of NLS is incorporated in substantial amounts into the sulfated GAGs of the developing rat and support the hypothesis that NLS is an important source of nutrient sulfate during rat development.
- Research Organization:
- New York State Institute for Basic Resources in Development Disability, Staten Island
- OSTI ID:
- 7108780
- Report Number(s):
- CONF-8606151-; TRN: 86-039021
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOPROTEINS
BIOSYNTHESIS
SULFATES
METABOLISM
LACTATION
LACTOSE
MILK
RATS
SULFUR 35
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques