Partial purification of the ATP-driven calcium pump of Streptococcus sanguis
ATP-dependent transport of calcium has been observed in several species of streptococci as uptake of /sup 45/Ca/sup 2 +/ into everted membrane vesicles. Membranes from Streptococcus sanguis and Streptococcus faecalis were solubilized with octyl-..beta..-D-glucoside or Triton X-100, and the extracts reconstituted into proteoliposomes containing Escherichia coli or soybean phospholipid. Calcium transport in reconstituted proteoliposomes was insensitive to the ionophores nigericin and valinomycin and was unaffected by the F/sub 0/F/sub 1/ inhibitor N,N'-dicyclohexylcarbodiimide. Uptake was inhibited by ortho-vanadate with a K/sub i/ in the micromolar range. These results demonstrate that the reconstituted transport activities are not the result of ATP-driven proton pumping via the F/sub 0/F/sub 1/ coupled to a calcium/proton antiporter and suggest that existence of a calcium translocating ATPase. Partial purification of the transport activity from Streptococcus sanguis has been achieved using density gradient centrifugation and FPLC.
- Research Organization:
- Univ. of Maryland School of Medicine, Baltimore
- OSTI ID:
- 7108609
- Report Number(s):
- CONF-8606151-; TRN: 86-039065
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:7; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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ATP-ASE
PURIFICATION
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
ATP
CALCIUM 45
CELL MEMBRANES
IMIDES
INHIBITION
LIPOSOMES
STREPTOCOCCUS
TRACER TECHNIQUES
VALINOMYCIN
VANADATES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HYDROLASES
INTERMEDIATE MASS NUCLEI
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ISOTOPES
MEMBRANES
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
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TRANSITION ELEMENT COMPOUNDS
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550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques