PDGF-induced receptor phosphorylation and phosphoinositide hydrolysis are unaffected by protein kinase C activation in mouse swiss 3T3 and human skin fibroblasts

Journal Article · Thu May 29 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91216-7· OSTI ID:7107098

Sturani, E; Vicentini, L M; Zippel, R; Toschi, L; Pandiella-Alonso, A; Comoglio, P M; Meldolesi, J

Short (1-10 min) pretreatment of intact cells with activators of protein kinase C (e.g. phorbol-12 myristate, 13-acetate, PMA) affects the activity of a variety of surface receptors (for growth factors, hormones and neurotransmitters), with inhibition of transmembrane signal generation. In two types of fibroblasts it is demonstrated that the PDGF receptor is unaffected by PMA. Exposure to PMA at concentrations up to 100 nM for 10 min failed to inhibit either one of the agonist-induced, receptor-coupled responses of PDGF: the autophosphorylation of receptor molecules at tyrosine residues, and the hydrolysis of membrane polyphosphoinositides. In contrast, the EGF receptor autophosphorylation (in A 431 cells) and the bombesin-induced phosphoinositide hydrolysis were readily inhibited by PMA.

• Research Organization: Univ. of Milano, Italy
• OSTI ID: 7107098
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 137:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
BIOCHEMICAL REACTION KINETICS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
HYDROLYSIS
PHOSPHOTRANSFERASES
CHEMICAL ACTIVATION
RECEPTORS
PHOSPHORYLATION
CELL MEMBRANES
EXPERIMENTAL DATA
INHIBITION
MICE
PROTEINS
SKIN
TYROSINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DATA
DECOMPOSITION
ENZYMES
ESTERS
HYDROXY ACIDS
INFORMATION
KINETICS
LIPIDS
LYSIS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
REACTION KINETICS
RODENTS
SOLVOLYSIS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
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