Intracellular processing of epidermal growth factor by early wound healing cells

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Surgery; (USA)

OSTI ID:7105772

Seyfer, A E; Nassaux, P; Emory, R; Wray, H L; Schaudies, R P ^[1]

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1. Walter Reed Army Medical Center, Washington, DC (USA)

Epidermal growth factor (EGF) is a potent 53-amino-acid residue polypeptide that has been implicated in normal wound healing. Although past studies have shown that locally applied EGF accelerates wound healing, these studies have not examined intracellular events related to the processing of the growth factor. The objective of this study was to characterize both initial and later postbinding intracellular processing of EGF by a responsive cell line (osteoblasts) that is important in the healing of wounds. Cloned mouse calvarial osteoblasts (MC-3TC-E1) were incubated with radiolabeled EGF, with and without preincubation with nonlabeled EGF, for specific time intervals. Cell-associated radioactivity was characterized by nondenaturing polyacrylamide gel electrophoresis. Results showed that EGF is processed as three distinct species and that the relative proportions of these species are altered at later time periods when compared with initial processing. The patterns, similar to those reported for human fibroblasts, indicate a possible common pathway for the mitogenic signal in cells associated with the early events of wound healing. In addition, these data represent the first direct evidence that preexposure of cells to nonlabeled EGF alters the processing of radiolabeled EGF. This is significant, because cells must be exposed to EGF for 5 to 8 hours to elicit a growth response. Such data may help to explain the lag phase of wound healing.

• OSTI ID: 7105772
• Journal Information: Surgery; (USA), Vol. 107:1; ISSN 0039-6060
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
GROWTH
GROWTH FACTORS
CHEMICAL COMPOSITION
WOUNDS
HEALING
BIOLOGICAL PATHWAYS
CELL CULTURES
ELECTROPHORESIS
EPIDERMIS
IODINE ISOTOPES
ISOTOPE DILUTION
MICE
ANIMAL TISSUES
ANIMALS
BIOLOGICAL RECOVERY
BODY
EPITHELIUM
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RECOVERY
RODENTS
SKIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques