Purification of PRL receptors from toad kidney: Comparisons with rabbit mammary PRL receptors

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:7101061

Dunand, M; Kraehenbuhl, J P; Rossier, B C; Aubert, M L ^[1]

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1. Univ. of Geneva School of Medicine (Switzerland) Univ. of Lausanne School of Medicine (Switzerland)

The binding characteristics of the prolactin (PRL) receptors present in toad (Bufo marinus) kidneys were investigated and compared to those of PRL receptors present in rabbit mammary glands. The molecular characteristics of the Triton X-100 solubilized renal and mammary PRL receptors were assessed by gel filtration and by migration analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after affinity labeling of the binding sites with {sup 125}I-human growth hormone. Similar results were obtained for both receptors. Partial purification of the toad PRL receptor could be achieved by affinity chromatography. The molecular weight of this purified receptor could be determined by analysis of SDS-PAGE. With the use of a polyclonal antiserum raised against a purified preparation of rabbit mammary PRL receptor, one or several antigenic epitope(s) could be identified on the core of the toad renal PRL receptor. In conclusion, although the structure and the biological role(s) of PRL have substantially changed during evolution, the receptor for this hormone has retained many of its structural features as could be assessed between an amphibian and a mammalian species on functionally different target tissues.

• OSTI ID: 7101061
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LTH
RADIORECEPTOR ASSAY
MEMBRANE PROTEINS
AUTORADIOGRAPHY
RECEPTORS
MOLECULAR BIOLOGY
STH
BIOLOGICAL EVOLUTION
CELL MEMBRANES
ELECTROPHORESIS
IMMUNE SERUMS
IODINE 125
KIDNEYS
MAMMARY GLANDS
MOLECULAR WEIGHT
PURIFICATION
RABBITS
TOADS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques